Stepwise Objectives    

Chromium Single Cell Multiome + Gene Expression provides a comprehensive, scalable multiomic approach for simultaneously profiling epigenomic landscape and gene expression in the same single nuclei. This is achieved by transposing nuclei in a bulk solution. Using a microfluidic chip, the nuclei are partitioned into nanoliter-scale Gel Beads-in-emulsion (GEMs). A pool of 736,000 10x Barcodes is sampled to separately and uniquely index the transposed DNA and cDNA of each individual nucleus. ATAC and gene expression (GEX) libraries are generated from the same pool of pre-amplified transposed DNA/cDNA and sequenced. The 10x Barcodes in each library type are used to associate individual reads back to the individual partitions, and thereby, to each individual nucleus.

GEM-X Epi Multiome provides a comprehensive, scalable multiomic approach for simultaneously profiling epigenomic landscape and gene expression in the same single nuclei. This is achieved by transposing nuclei in a bulk solution. Using a microfluidic chip, the nuclei are partitioned into nanoliter-scale Gel Beads-in-emulsion (GEMs). A pool of ~750,000 10x Barcodes is sampled to separately and uniquely index the transposed DNA and cDNA of each individual nucleus. ATAC and gene expression (GEX) libraries are generated from the same pool of pre-amplified transposed DNA/cDNA and sequenced. The 10x Barcodes in each library type are used to associate individual reads back to the individual partitions, and thereby, to each individual nucleus.