GEM-X Epi Multiome Reagents

10x reagents are listed in the same order as their placement in the kits.

Refer to SDS for handling and disposal information.

GEM-X Epi Multiome, 16 samples PN-1000979

GEM-X Epi Multiome GEM Kit 16 rxns, PN-1000945 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Barcoding Reagent C	2001558		Barcoding Enzyme B	2001562
	Template Switch Oligo B	2001027		Reducing Agent B	2000087
	Cleanup Buffer	2000088		Cleanup Buffer	2000088
	Quenching Agent	2001679	-	empty	-
-	empty	-	-	empty	-

GEM-X Epi Multiome AMP Kit 16 rxns, PN-1000947 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Pre-Amp Mix	2000270		Pre-Amp Primers	2000271
	Amp Mix	2000047		Amp Mix	2000047
	cDNA Primers	2000089		SI-PCR Primer B	2000128
-	empty	-	-	empty	-
-	empty	-	-	empty	-

GEM-X

Multiome Gel Bead Kit
16 rxns, PN-1000943

Shipped on dry ice
Store at -80°C

	#	PN
Single Cell Multiome Gel Bead	2	2001514

Dynabeads™ MyOne™ SILANE
PN-2000048

Shipped at ambient temperature
Store at 4°C

	#	PN
Dynabeads MyOne SILANE	1	2000048

GEM-X Epi Multiome ATAC Kit, 16 rxns PN-1000949

GEM-X Epi Multiome ATAC Kit 16 rxns, PN-1000949 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	20X Nuclei Buffer	2000207		20X Nuclei Buffer	2000207
	ATAC Buffer C	2001581		ATAC Enzyme B	2001582
	RNase Inhibitor 40x	2001488	-	empty	-
-	empty	-	-	empty	-
-	empty	-	-	empty	-

Library Construction Kit C, 16 rxns PN-1000694*

Library Construction Kit C 16 rxns, PN-1000694 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Fragmentation Enzyme	2000090		Fragmentation Buffer	2000091
	Ligation Mix	2001109		DNA Ligase	220110
	Library Amp Mix	2000531	-	empty	-
-	empty	-	-	empty	-
-	empty	-	-	empty	-

*Alternatively, use Library Construction Kit C, Automated 24 rxns/ Manual 32 rxns PN-1000774

This document describes the use of this kit for manual gene expression library construction. For details on using this kit for automated library construction, refer to Supplemental User Guide Library Construction Kit C (CG000791).

Library Construction Kit C Automated 24 rxns / Manual 32 rxns, PN-1000774 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Fragmentation Enzyme	2001315		Fragmentation Buffer	2001316
	Ligation Mix	2001317		DNA Ligase	2001318
	Library Amp Mix	2001319	-	empty	-
-	empty	-	-	empty	-
-	empty	-	-	empty	-

GEM-X Epi Multiome, 4 samples PN-1000980

GEM-X Epi Multiome GEM Kit 4 rxns, PN-1000946 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Barcoding Reagent C	2001558		Barcoding Enzyme B	2001562
	Template Switch Oligo B	2001027		Reducing Agent B	2000087
	Cleanup Buffer	2000088	-	empty	-
	Quenching Agent	2001679	-	empty	-
-	empty	-	-	empty	-

GEM-X Epi Multiome AMP Kit 4 rxns, PN-1000948 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Pre-Amp Mix	2000270		Pre-Amp Primers	2000271
	Amp Mix	2000047	-	empty	-
	cDNA Primers	2000089		SI-PCR Primer B	2000128
-	empty	-	-	empty	-
-	empty	-	-	empty	-

GEM-X

Multiome Gel Bead Kit
4 rxns, PN-1000944

Shipped on dry ice
Store at -80°C

	#	PN
GEM-X Multiome Gel Beads	0.5	2001514

Dynabeads™ MyOne™ SILANE
PN-2000048

Shipped at ambient temperature
Store at 4°C

	#	PN
Dynabeads MyOne SILANE	1	2000048

GEM-X Epi Multiome ATAC Kit, 4 rxns PN-1000950

GEM-X Epi Multiome ATAC Kit 4 rxns, PN-1000950 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	20X Nuclei Buffer	2000207		20X Nuclei Buffer	2000207
	ATAC Buffer C	2001581		ATAC Enzyme B	2001583
	RNase Inhibitor 40x	2001488	-	empty	-
-	empty	-	-	empty	-
-	empty	-	-	empty	-

Library Construction Kit C, 4 rxns PN-1000689

Library Construction Kit C 4 rxns, PN-1000689 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Fragmentation Enzyme	2000104		Fragmentation Buffer	2000091
	Ligation Mix	2001109		DNA Ligase	220131
-	empty	-	-	empty	-
-	empty	-	-	empty	-
-	empty	-	-	empty	-

GEM-X Epi Multiome Chip Kit, 4 chips PN-1000951

Chromium GEM-X
Partitioning Oil B

Shipped at ambient temperature
Store at ambient temperature

		#	PN
	Partitioning Oil B	6	2001213

Chromium GEM-X
Recovery Agent

Shipped at ambient temperature
Store at ambient temperature

		#	PN
	Recovery Agent	6	220016

GEM-X Epi Multiome
Chip & Gaskets

Shipped at ambient temperature
Store at ambient temperature

	#	PN
GEM-X MO Chip	4	2001485
X/iX Chip Gasket, 2-pack	2	3000656

Nuclei Isolation Kit with RNase Inhibitor v2, PN-1001101

RNase Inhibitor Kit v2, PN-1001099

RNase Inhibitor Kit v2 PN-1001099 Shipped on dry ice; Store at -20°C
Cap Color	Reagent	PN	Cap Color	Reagent	PN
	Reducing Agent B	2000087		20x Nuclei Buffer	2000207
	20x Nuclei Buffer	2000207	-	empty	-
	RNase Inhibitor 40x	2001488		RNase Inhibitor 40x	2001488
	RNase Inhibitor 40x	2001488		RNase Inhibitor 40x	2001488
	RNase Inhibitor 40x	2001488		RNase Inhibitor 40x	2001488

RNase Inhibitor 40x, Kit PN-1000887 (tube PN-2001488) can also be purchased separately

Single Index Kit N Set A, 96 rxns PN-1000212

Single Index Kit N Set A
96 rxns, PN-1000212

Shipped on dry ice
Store at −20°C

	#	PN
Single Index Plate N Set A	1	3000427

Dual Index Kit TT Set A, 96 rxns PN-1000215

Dual Index Kit TT Set A
96 rxns, PN-1000215

Shipped on dry ice
Store at −20°C

	#	PN
Dual Index Plate TT Set A	1	3000431

Reagent Kit Summary

Orderable Kit	PN	Kits	PN	Quantity
GEM-X Epi Multiome, 16 samples	1000979	GEM-X Epi Multiome Gel Bead Kit, 16 rxns	1000943	1
		GEM-X Epi Multiome GEM Kit, 16 rxns	1000945	1
		GEM-X Epi Multiome Amp Kit, 16 rxns	1000947	1
		Dynabeads™ MyOne™ SILANE	2000048	1
		GEM-X Epi Multiome ATAC Kit, 16 rxns	1000949	1
		Library Construction Kit C, 16 rxns	1000694 (orderable separately)	1
GEM-X Epi Multiome, 4 samples	1000980	GEM-X Epi Multiome Gel Bead Kit, 4 rxns	1000944	1
		GEM-X Epi Multiome GEM Kit, 4 rxns	1000946	1
		GEM-X Epi Multiome Amp Kit, 4 rxns	1000948	1
		Dynabeads™ MyOne™ SILANE	2000048	1
		GEM-X Epi Multiome ATAC Kit, 4 rxns	1000950	1
		Library Construction Kit C, 4 rxns	1000689
GEM-X Epi Multiome Chip Kit, 4 chips	1000951	GEM-X Epi Multiome Chip Kit, 4 chips	1000951	1
Nuclei Isolation Kit with RNase Inhibitor v2	1001101	Chromium Nuclei Isolation Consumables Kit, 16 rxns	1000448	1
		Chromium Nuclei Isolation Reagents Kit, 16 rxns	1000447	1
		RNase Inhibitor v2	1001099	1
RNase Inhibitor 40x, Kit PN-1000887 (tube PN-2001488) can also be purchased separately
Single Index Kit N Set A, 96 rxns	1000212	Single Index Kit N Set A, 96 rxns	1000212	1
Dual Index Kit TT Set A, 96 rxns	1000215	Dual Index Kit TT Set A, 96 rxns	1000215	1