Epi Multiome

CG001689

Individual Transp.

Scalable Transp.

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Step 5: Pre Amplification PCR

Barcoded transposed DNA and barcoded full length cDNA from poly-adenylated mRNA are amplified via PCR to fill gaps and for generating sufficient mass for library construction. The pre-amplified product is used as input for both ATAC library construction and cDNA amplification for gene expression library construction.

Pooled pre-amplification PCR