Step 8: Gene Expression Library Construction

Enzymatic fragmentation and size selection are used to optimize the cDNA amplicon size. P5, P7, i7 and i5 sample indexes, and TruSeq Read 2 (read 2 primer sequence) are added via End Repair, A-tailing, Adaptor Ligation, and PCR. The final gene expression libraries contain the P5 and P7 primers used in Illumina® amplification.

cDNA Amplification & Gene Expression Library Construction