Step 1 or Step 2: Transposition
Nuclei suspensions are incubated in a Transposition Mix that includes a Transposase. The Transposase enters the nuclei and preferentially fragments the DNA in open regions of the chromatin. Simultaneously, adapter sequences are added to the ends of the DNA fragments. Depending on starting nuclei input and experimental goals, choose either Individual or Scalable Transposition as per the Transposition Selection Guidelines.