Sample Split Overview
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80 μl pre-amplified, SPRI cleaned sample derived at end of step 5.3 includes barcoded transposed DNA fragments and barcoded cDNA.
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The sample is divided and used as input for two separate steps.
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40 μl sample is used for ATAC Library Construction (step 6)
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35 μl sample is used for cDNA Amplification (step 7).
The amplified cDNA will be used for Gene Expression Library Construction.
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