5.3 Pre Amplification SPRI Cleanup

  1. Vortex the SPRIselect reagent until fully resuspended. Add 160 μl SPRIselect reagent to each sample. Pipette mix 15x.

  2. Incubate 5 min at room temperature.

  3. Centrifuge briefly. Place on the magnet•High until the solution clears.

  4. Remove the supernatant.

  5. Add 300 μl 80% ethanol to the pellet. Wait 30 sec.

  6. Remove the ethanol.

  7. Add 200 μl 80% ethanol to the pellet. Wait 30 sec.

  8. Remove the ethanol.

  9. Centrifuge briefly. Place on the magnet•Low.

  10. Remove any remaining ethanol.

  11. Remove the tube strip from the magnet. Air dry for 2 min. Add 80.5 μl Buffer EB.

  12. Pipette mix (pipette set to 75 μl) without introducing bubbles.

  13. Incubate 2 min at room temperature.

  14. Centrifuge briefly. Place on the magnet•High until the solution clears.

  15. Transfer 80 μl sample to a new tube strip.

  16. Store at 4°C for up to 72 h or at −20°C for long term storage, or proceed to the next step.