5.2 Pre Amplification PCR    

  1. Incubate in a thermal cycler with the following protocol.

    Lid Temperature

    Reaction Volume

    Run Time
    hh:mm:ss

    105°C

    100 µl

    30 min

    Step

    Temperature

    Time

    1

    72°C

    00:05:00

    2

    98°C

    00:03:00

    3

    98°C

    00:00:20

    4

    63°C

    00:00:30

    5

    72°C

    00:01:00

    Go to step 3

    repeat 5X (Total 6 cycles*)

    6

    72°C

    00:01:00

    7

    4°C

    Hold

    *Do not modify cycle number

  2. Store at 4°C for up to 18 h or proceed to the next step.