5.0 Get Started
|
Action |
Item |
10x PN |
Preparation & Handling |
Storage |
|
|---|---|---|---|---|---|
| Equilibrate to Room Temperature | |||||
|
|
|
Pre-Amp Primers |
2000271 |
Vortex, centrifuge briefly. |
−20°C |
|
Beckman Coulter SPRIselect Reagent |
- |
Manufacturer’s recommendations. |
- |
||
|
Place on Ice |
|||||
|
|
|
Pre-Amp Mix |
2000270/ 2000274 |
Thaw completely on ice. Gently pipette mix, centrifuge briefly. |
−20°C |
| Obtain | |||||
|
|
Qiagen Buffer EB |
- |
- |
Ambient |
|
|
10x Magnetic Separator |
230003/ 2001212 |
See Tips & Best Practices. |
Ambient |
|
|
|
Prepare 80% Ethanol Prepare 10 ml for 8 reactions |
- |
Prepare fresh. |
Ambient |
|