5.1 Prepare Pre Amplification Mix

  1. Prepare Pre Amplification Mix on ice. Pipette mix 10x and centrifuge briefly.

     

    Pre Amplification Mix
    Add reagents in the order listed

    PN

    1X (μl)

    4X +

    10% (μl)

    8X +

    10% (μl)

    Pre-Amp Mix

    2000270/ 2000274

    50.0

    220.0

    440.0

    Pre-Amp Primers

    2000271

    4.0

    17.6

    35.2
     

    Total

    -

    54.0

    237.6

    475.2

  2. Add 54 μl Pre Amplification Mix to each sample. Pipette mix and centrifuge briefly.