5.1 Prepare Pre Amplification Mix

  1. Prepare Pre Amplification Mix on ice. Pipette mix 10x and centrifuge briefly.

    2

     

    Fixed percentage added to total to help with pipetting

    Master Mix
    Add reagents in the order listed

    PN

    Volume for Master Mix (μl)

    Scaled Volume (μl)

    Pre-Amp Mix

    2000270/ 2000274

    50

     

    Pre-Amp Primers

    		 2000271 4

     

    Total

    		 

    54

     

  2. Add 54 μl Pre Amplification Mix to each sample. Pipette mix and centrifuge briefly.