8.4 Post Ligation Cleanup – SPRIselect

  1. Vortex to resuspend SPRIselect Reagent. Add 80 μl SPRIselect Reagent (0.8X) to each sample. Pipette mix 15x (pipette set to 150 μl).

  2. Incubate 5 min at room temperature.

  3. Place on the magnet•High until the solution clears.

  4. Remove the supernatant.

  5. Add 200 μl 80% ethanol to the pellet. Wait 30 sec.

  6. Remove the ethanol.

  7. Repeat steps e and f for a total of 2 washes.

  8. Centrifuge briefly. Place on the magnet•Low.

  9. Remove any remaining ethanol. Air dry for 2 min. DO NOT exceed 2 min as this will decrease elution efficiency.

  10. Remove from the magnet. Add 30.5 μl Buffer EB. Pipette mix 15x.

  11. Incubate 2 min at room temperature.

  12. Place on the magnet•Low until the solution clears.

  13. Transfer 30 μl sample to a new tube strip.