8.0 Get Started

Action

  

Item

10x PN

Preparation & Handling

Storage
Equilibrate to Room Temperature

Fragmentation Buffer

2000091/
2001316

Vortex, verify no precipitate, centrifuge briefly.

−20°C
Ligation Mix 2001109/
2001317		 Thaw, vortex, verify no precipitate, centrifuge. −20°C
 

Dual Index Plate TT Set A

3000431

-

−20°C
 

Beckman Coulter SPRIselect Reagent

-

Manufacturer’s recommendations.

-
 

Agilent TapeStation Screen Tape and Reagents (if used for QC)

 

Manufacturer’s recommendations.

 
 

Agilent Bioanalyzer High Sensitivity kit (if used for QC)

-

Manufacturer’s recommendations.

-
 

DNA High Sensitivity Reagent Kit (if LabChip used for QC)

-

Manufacturer’s recommendations.

-
Place on Ice

Fragmentation Enzyme

2000090/

2000104/
2001315

Centrifuge briefly.

−20°C

DNA Ligase

220110/

220131/2001318

Centrifuge briefly.

−20°C

Library Amp Mix

2000531/2001319

Centrifuge briefly.

−20°C
 

KAPA Library Quantification Kit for Illumina Platforms

-

Manufacturer’s recommendations.

-
Obtain

 

Qiagen Buffer EB

-

-

Ambient
 

10x Magnetic Separator

230003

See Tips & Best Practices.

Ambient
 

Prepare 80% Ethanol

Prepare 20 ml for 8 reactions

-

Prepare fresh.

Ambient