8.7 Post Library Construction QC

Run 1 μl sample at 1:10 dilution on an Agilent Bioanalyzer High Sensitivity chip.

Determine the average fragment size from the Bioanalyzer trace. This will be used as the insert size for library quantification.

Alternate QC Methods

See Appendix for representative traces:

• Agilent TapeStation Traces

• LabChip Traces

See Appendix for Post Library Construction Quantification