3.1 Prepare Master Mix

  1. Prepare Master Mix on ice. Pipette mix 10x and centrifuge briefly.

    2

     

    Fixed percentage added to total to help with pipetting

    Master Mix

    Add reagents in the order listed

    		PN

    Volume for Master Mix (μl)

    Scaled Volume (μl)

    Barcoding Reagent C

    2001558

    29.5

     

    Template Switch Oligo B

    		 2001027

    1.1

     

    Reducing Agent B

    		 2000087

    1.9

     

    Barcoding Enzyme B

    2001562/

    2001561

    7.5

     

    Total