3.1 Prepare Master Mix
-
Prepare Master Mix on ice. Pipette mix 10x and centrifuge briefly.
Master Mix
Add reagents in the order listed
PN
1X (µl)
4X +
10% (µl)
8X +
10% (µl)
Barcoding Reagent C
2001558
29.5
129.8 259.6 
Template Switch Oligo B
2001027
1.1
4.8 9.7 
Reducing Agent B
2000087
1.9
8.3 16.7
Barcoding Enzyme B
2001562/
2001561
7.5
33.0 66.0 Total
-
40.0
176.0 352.0