3.0 Get Started
Firmware version 2.0.0 or higher is required in the Chromium X Series instrument to run the GEM-X chips.
|
Action |
Item |
10x PN |
Preparation & Handling |
Storage |
|
|---|---|---|---|---|---|
| Equilibrate to Room Temperature | |||||
|
|
GEM-X Multiome Gel Beads |
2001514 |
Equilibrate to room temperature 30 min before loading the chip. |
−80°C |
|
|
|
Template Switch Oligo B |
2001027 |
Centrifuge briefly, resuspend in 52.8 µl Low TE Buffer. Vortex 15 sec at maximum speed, centrifuge briefly, leave at room temperature for ≥ 30 min. After resuspension, store at −80°C. Thaw at room temperature for ≥ 30 minutes in subsequent uses |
−20°C |
|
|
|
Reducing Agent B |
2000087 |
Thaw, vortex, verify no precipitate, centrifuge briefly. |
−20°C |
|
|
|
Barcoding Reagent C |
2001558 |
Thaw, vortex, verify no precipitate, centrifuge briefly. |
−20°C |
|
|
|
Quenching Agent | 2001679 | Equilibrate to room temperature during GEM Incubation step | −20°C |
| Place on Ice | |||||
|
|
|
Barcoding Enzyme B |
2001562/ 2001561 |
Centrifuge briefly. Maintain on ice. Gently pipette mix right before use. Store at −20°C immediately after use. |
−20°C |
| Obtain | |||||
|
|
|
Partitioning Oil B |
2001213 |
- |
Ambient |
|
|
Low TE Buffer |
- |
Manufacturer’s recommendations. |
- |
|
|
|
GEM-X MO Chip |
2001485 |
See Tips & Best Practices. |
Ambient |
|
|
|
10x Gasket |
3000656 |
See Tips & Best Practices. |
Ambient |
|
|
|
10x Vortex Adapter |
330002 |
See Tips & Best Practices. |
Ambient |
|
|
|
Chromium X Series Chip Holder |
3000598 |
See Tips & Best Practices |
Ambient |
|
|
|
50% glycerol solution If using <8 reactions |
- |
See Tips & Best Practices. |
- |
|
