Sample Indices in Sample Index PCR

  • Choose the appropriate sample index sets to ensure that no sample indices overlap in a multiplexed sequencing run.

  • Each well in the Single Index plate N, Set A contains a unique mix of 4 oligos.

  • Each well in the Dual Index Plate TT Set A contains a unique i7 and a unique i5 oligonucleotide.

  • Verify and use the specified index plate only. DO NOT use the plates interchangeably.