General Reagent Handling

• Fully thaw and thoroughly mix reagents before use.

• Keep all enzymes and Master Mixes on ice during setup and use. Promptly move reagents back to the recommended storage.

• Calculate reagent volumes with 10% excess of reaction volumes.

• Cover Partitioning Oil tubes and reservoirs to minimize evaporation.

• Thoroughly mix samples with the beads during bead-based cleanup steps.