GEM-X Chip Handling
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Minimize exposure of reagents, chips, and gaskets to sources of particles and fibers, laboratory wipes, frequently opened flip-cap tubes, clothing that sheds fibers, and dusty surfaces.
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After removing the chip from the sealed bag, use in ≤24 h.
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Execute steps without pause or delay, unless indicated. When using multiple chips, load, run, and collect the content from one chip before loading the next.
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Fill all unused input wells in rows labeled 1, 2, and 3 on a chip with an appropriate volume of 50% glycerol solution before loading the used wells. DO NOT add glycerol to the wells in the top NO FILL row.
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Avoid contacting the bottom surface of the chip with gloved hands and other surfaces. Frictional charging can lead to inadequate priming of the channels, potentially leading to wetting failures.
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Minimize the distance that a loaded chip is moved to reach the compatible Chromium X series instrument (X/iX).
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Keep the chip horizontal to prevent wetting the gasket with oil, which depletes the input volume and may adversely affect the quality of the resulting emulsion.