4.2 Post GEM Incubation Cleanup – SPRIselect

  1. Vortex the SPRIselect reagent until fully resuspended. Add 90 μl SPRIselect reagent to each sample. Pipette mix (pipette set to 130 μl) thoroughly.

  2. Incubate 5 min at room temperature.

  3. Centrifuge briefly. Place on the magnet•High until the solution clears.

  4. Remove the supernatant.

  5. Add 200 μl 80% ethanol to the pellet. Wait 30 sec.

  6. Remove the ethanol.

  7. Repeat steps e and f for a total of 2 washes.

  8. Centrifuge briefly. Place on the magnet•Low.

  9. Remove any remaining ethanol.

    Residual ethanol can inhibit Pre-Amplification PCR and impact assay performance.

  1. Remove the tube strip from the magnet. Air dry for 1 min. Immediately add 46.5 μl Buffer EB.

  2. Pipette mix (pipette set to 45 μl) without introducing bubbles.

  3. Incubate 2 min at room temperature.

  4. Centrifuge briefly. Place on the magnet•Low until the solution clears.

  5. Transfer 46 μl sample to a new tube strip.

    Residual SPRI beads can inhibit Pre-Amplification PCR and impact assay performance.