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Item

10x PN

Preparation & Handling

Storage
Equilibrate to Room Temperature

Reducing Agent B

2000087

Thaw, vortex, verify no precipitate, centrifuge briefly.

−20°C
 

Nuclease-free Water

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-

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Dynabeads MyOne SILANE

2000048

Vortex thoroughly (≥30 sec)

immediately before adding to the mix. If still clumpy, pipette mix to resuspend completely. DO NOT centrifuge before use.

4°C
 

Beckman Coulter SPRIselect Reagent

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Manufacturer’s recommendations.

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Thaw at 65°C

Cleanup Buffer

2000088

Thaw for 10 min at

65°C at max speed on a thermomixer. Verify there are no visible crystals.

Cool to room temperature.

−20°C
Obtain

 

Recovery Agent

220016

-

Ambient
 

Qiagen Buffer EB

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Manufacturer’s recommendations.

-
 

Bio-Rad 10% Tween 20

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Manufacturer’s recommendations.

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10x Magnetic Separator

230003/ 2001212

-

Ambient
 

Prepare 80% Ethanol

Prepare 10 ml for 8 reactions

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Prepare fresh in nuclease-free water.

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