7.0 Get Started
|
Action |
Item |
10x PN |
Preparation & Handling |
Storage |
|
|---|---|---|---|---|---|
| Equilibrate to Room Temperature | |||||
|
|
|
cDNA Primers |
2000089 |
Vortex, centrifuge briefly. |
−20°C |
|
|
Beckman Coulter SPRIselect Reagent |
- |
Manufacturer’s recommendations. |
- |
|
|
|
Agilent Bioanalyzer High Sensitivity Kit (if used for QC and quantification) |
- |
Manufacturer’s recommendations. |
- |
|
|
|
Agilent TapeStation ScreenTape and Reagents (if used for QC and quantification) |
- |
Manufacturer’s recommendations. |
- |
|
|
|
Qubit dsDNA HS Assay Kit (if used for QC and quantification) |
- |
Manufacturer’s recommendations. |
- |
|
| Place on ice | |||||
|
|
|
Amp Mix |
2000047 |
Vortex, centrifuge briefly. |
−20°C |
| Obtain | |||||
|
|
Qiagen Buffer EB |
- |
Manufacturer’s recommendations. |
- |
|
|
|
10x Magnetic Separator |
230003 |
- |
Ambient |
|
|
|
Prepare 80% Ethanol - |
- |
- |
||