Sequencing Libraries

Chromium Single Cell Multiome ATAC libraries comprise double stranded DNA with standard Illumina® paired-end constructs which begin with P5 and end with P7. Sequencing these libraries produces a standard Illumina® BCL data output folder that includes paired-end Read 1N and Read 2N used for sequencing the DNA insert, 8 bp sample index in the i7 read, and 24 bp in the i5 read to cover the 16 bp 10x Barcode and 8 bp Spacer.

Chromium Single Cell Multiome Gene Expression libraries comprise cDNA insert with standard Illumina® paired-end constructs which begin with P5 and end with P7. Sequencing these libraries produces a standard Illumina® BCL data output folder. TruSeq Read 1 is used to sequence 16 bp 10x Barcodes and 12 bp UMI, while 10 bp i5 and i7 sample index sequences are the sample index reads. TruSeq Read 2 is used to sequence the insert.