ATAC Library Loading
Once quantified and normalized, libraries should be denatured and diluted as recommended for Illumina® sequencing platforms. Refer to Illumina® documentation for denaturing and diluting libraries. Refer to the 10x Genomics Support website for more information.
|
Instrument |
Loading Concentration (pM) |
PhiX (%) |
|---|---|---|
|
MiSeq™ |
10 |
1 |
|
NextSeq™ 500/550 |
1.5 |
1 |
|
NextSeq™ 1000/2000 |
650 |
1 |
|
HiSeq™ 2500 (RR) |
10 |
1 |
|
HiSeq™ 4000 |
180 |
1 |
|
NovaSeq™ 6000 Standard & Xp workflow |
150 |
1 |
|
NovaSeq™ X Series |
150-200 | 1 |