6.0 Get Started

Action

  

Item

10x PN

Preparation & Handling

Storage
Equilibrate to Room Temperature

 

Sample Index Plate N, Set A

3000427

-

−20°C
 

Beckman Coulter SPRIselect Reagent

-

Manufacturer’s recommendations.

-
 

Agilent Bioanalyzer DNA kit (if used for QC)

-

Manufacturer’s recommendations.

-
 

DNA High Sensitivity Reagent Kit (if LabChip used for QC)

-

Manufacturer’s recommendations.

-
Place on Ice

SI-PCR Primer B

2000128

Vortex, centrifuge briefly.

−20°C

Amp Mix

2000047

Gently pipette mix, centrifuge briefly.

−20°C
 

KAPA Library Quantification Kit for Illumina® Platforms

-

Manufacturer’s recommendations.

-
Obtain

 

Qiagen Buffer EB

-

-

Ambient
 

10x Magnetic Separator

230003

See Tips & Best Practices.

Ambient
 

Prepare 80% Ethanol

Prepare 10 ml for 8 reactions

-

Prepare fresh.

Ambient