2.2 Isothermal Incubation

  1. Incubate in a thermal cycler using the following protocol.

    Lid Temperature

    Reaction Volume

    Run Time
    hh:mm:ss

    50°C

    25 µl

    30 min

    Step

    Temperature

    Time

    Incubate

    37°C

    00:30:00 (for cell suspensions like PBMC)
    00:60:00 (for tissue)

    Hold

    4°C

    00:00:20

    During the incubation, prepare Transposition Wash Buffer as outlined in Post Transposition Wash.

  2. Immediately proceed to the next step.